银杏叶提取物磷脂酰胆碱复合物对缺血大鼠学习记忆障碍的影响

The protective effects of Ginkgo biloba extract phosphatidylcholide complex on the learning and memory impairment in cerebral ischemia rats

目的 观察银杏叶提取物磷脂酰胆碱复合物(Gb-PC)对血管性痴呆(VD)大鼠学习记忆功能的影响,并探讨其相关机制。方法 制备VD模型,采用避暗法及水迷宫法评价Gb-PC对VD模型大鼠学习记忆的影响,并测定大鼠海马组织中单胺氧化酶(MAO)及超氧化物歧化酶(SOD)活性,丙二醛(MDA)含量。结果Gb-PC可以明显改善VD大鼠的学习记忆功能,同时可不同程度抑制海马组织中MAO活性,增强SOD活性,减少MDA含量。结论 Gb-PC对VD大鼠学习记忆功能有一定的改善作用,其机制可能与提高脑组织中抗氧化酶活性,减轻生物膜脂质过氧化损伤以及抑制单胺氧化酶活性有关。

OBJECTIVE To study the protective effects of Ginkgo biloba extract phosphatidylcholide complex (Gb-PC) on the learning and memory impairment in rats of vascular dementia (VD) , and discuss the relevant mechanisms. METHODS VD model rats were made by repeated cerebral ischemia-reperfusion. Step-through and morris water maze experiments were used to detect the learning and memory ability. The activities of monoamine oxidase (MAO) and superoxide dismutase (SOD) and the contents of malonyldialdehyde (MDA) in the hippocampus tissues were measured to explore the possible mechanisms. RESULTS Gb-PC obviously improved the learning and memory ability of VD rats, and lowered the content of MDA, restored the lowered activity of SOD and inhibited the activity of MAO in the hippocampus tissues. CONCLUSION It was concluded that Gb-PC, to a certain degree, improved the learning and memory ability of VD rats. The relevant mechanisms may be related to increasing antioxidase activities, decreasing of damage of lipid peroxidative to biomembrane and inhibiting the activity of MAO in the hippocampus tissues.