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藻蓝蛋白裂合酶基因的表达与酶活性研究 被引量：8

The expression of PcE/F of phycocyanin operon and the study of lyase PcE/F activity of Mastigocladuc laminosus
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摘要 为了比较研究层理鞭枝藻藻蓝蛋白裂合酶PcE/F与藻红蓝蛋白裂合 /异构酶PecE/F的结构与功能 ,将藻蓝蛋白裂合酶PcE/F氨基酸序列与其他蓝藻的相应序列进行比较 ,并进行大量表达 ,将表达的裂合酶PcE/F用于藻蓝胆素 (PCB)与藻蓝蛋白α 亚基 (α PC)脱辅基蛋白 (PcA)的体外重组 ,得到天然活性的α PC ,从而表明层理鞭枝藻中 pcE/F所编码的蛋白质是α PC生物合成的裂合酶 . To study the structure-function relationships of phy c ocyanin lyase PcE/F and the phycoerythrocyanin lyase-isomerase PecE/F, the homo logy of amino acid sequences of PcE/F from several species of cyanobacteria was analyzed. Based on the analyses, the lyase PcE/F of Mastigocladuc laminosus was re-cloned and overexpressed. By reconstitutions in vitro using the overexpr essed lyase PcE/F, it was shown that PcE/F from Mastigocladuc laminosus was the specific enzyme for biosynthesis of phycocyanin α-subunit.
作者 李梅 武栋 周明 赵开弘
机构地区 华中科技大学环境科学与工程学院 华中科技大学生命科学与技术学院
出处 《华中科技大学学报（自然科学版）》 EI CAS CSCD 北大核心 2004年第2期77-79,共3页 Journal of Huazhong University of Science and Technology(Natural Science Edition)
基金 国家自然科学基金资助项目 ( 30 2 70 32 6 90 2 0 10 0 1)
关键词 藻蓝蛋白 PcE/F 重组 phycocyanin PcE/F layase reconstitution
分类号 Q814 [生物学—生物工程]
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共引文献4

同被引文献72

  • 1陈芳,周明,赵岩,滕吟,朱菁萍,赵开弘.鱼腥藻PCC7120藻蓝蛋白α亚基体内重组研究[J].武汉植物学研究,2006,24(5):387-391. 被引量：6
  • 2杨立红,王晓洁,钟旭升,冯培勇,李小青.鱼腥藻藻蓝蛋白的抗氧化作用[J].食品科学,2006,27(12):208-213. 被引量：15
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引证文献8

二级引证文献10

华中科技大学学报（自然科学版）
2004年 第2期

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