摘要
以荧光猝灭原理为基础 ,以钌Ⅱ 邻菲咯啉为荧光试剂制备荧光薄膜 ,以醋酸纤维素为胆固醇氧化酶固定化载体 ,研制了胆固醇光纤生物传感器。利用相移法原理 ,采用锁相放大技术 ,实现了对胆固醇溶液中溶解氧和胆固醇含量的检测。该传感器具有较高的灵敏度 ,快的响应时间和较好的稳定性。
In this work, a fiber optical biosensor for detecting cholesterol was developed based on fluorescence quenching. Ru(phen)32+ was used as a fluorescent reagent to prepare fluorescent membrane, and cellulose acetate was used as a carrier of the immobilized cholesterol oxidase. The dissolved oxygen and cholesterol content in cholesterol solution were detected by means of phase-shifting and phase lock means. This sensor has high sensitivity, Short response time and good stabilization.
出处
《中国生物医学工程学报》
EI
CAS
CSCD
北大核心
2004年第1期44-48,共5页
Chinese Journal of Biomedical Engineering
基金
高等学校博士学科点专项基金项目资助 ( 980 4970 3 )
关键词
光纤传感器
胆固醇
荧光猝灭
相移法
Biocatalysts
Biosensors
Cellulose
Cholesterol
Fiber optics
Fluorescence
Oxidation
Quenching
Sensitivity analysis
