异烟腙类衍生物TJU103体外对人T淋巴细胞活化功能的影响

Effects of TJU103 on T Lymphoytes activation in vitro

目的 探讨异烟腙类衍生物 TJU10 3体外对人 T淋巴细胞活化功能的影响。方法 体外建立 HL A半相合供、受者间单向混合淋巴细胞培养 (ML C)体系 ,应用 TJU10 3阻断 CD4 T细胞激活的抗原信号通路 ,利用MTT法检测 T淋巴细胞的增殖和细胞毒活性 ,酶联免疫吸附法 (EL ISA)检测对 T淋巴细胞分泌细胞因子的影响。结果 TJU10 3能明显降低 HL A半相合供、受者间混合淋巴细胞增殖反应 ,以及肿瘤坏死因子 α(TNF- α)和 γ干扰素 (IFN-γ)等炎性细胞因子的分泌 (P <0 .0 1) ,但不影响供者 T细胞对原代白血病细胞的杀伤效应 (P >0 .0 5 )。结论 TJU10 3能特异性地与 CD4 T淋巴细胞结合 ,阻断 CD4 T淋巴细胞激活的抗原信号通路 ,在明显降低 T淋巴细胞增殖反应和炎性细胞因子分泌的同时 ,不影响其细胞毒作用发挥 。

Objective To investigate the effects of TJU103 on T lymphocytes activation in vitro.Methods MTT and ELISA assays were used respectively to detect the proliferation,cytotoxicity and the cytokine production of donor T lymphocytes blocked by TJU103.Results There was no differences in the ability of anti-leukemia between each group,but there was significant difference in the prohibition of T-cell proliferation and the cytokine production between groups of TJU103.Conclusion TJU103 may prohibit the T-cell proliferation and the cytokine production significantly but the ability of anti-leukemia is not influenced.