白屈菜中异喹啉生物碱的高效薄层分离及荧光扫描法

HPTLC SEPARATION AND FLUORODENSITOMETRIC DETERMINATION OF ISOQUINOLINE ALKALOIDS IN CHELIDONINE MAJUS L.

本文在高效硅胶板(10×10cm)上用一种展开剂,首次分离了白屈菜中八种异喹啉生物碱,即白屈菜碱(chelidonine)、黄连碱(coptisine)、四氢黄连碱(tetrahydrocoptisine)、普托品(protopine)、6-甲氧基二氢白屈菜红碱(6-methoxydihydrochelerthrine)、6-甲氧基二氢血根碱(6-methoxydihydrosanguinarine)、二氢血根碱(dihydrosanguinarine)及小檗碱(berberine),经薄层荧光衍生化后,用荧光扫描法测定了这些生物碱的含量。本实验还在薄层荧光衍生化、荧光强度的稳定及增强等方面进行了研究。

The quantitative analysis of isoquinoline alkaloids in Chelidoninemajus L. was investigated and a HPTLC method has been established. Using this method,we separated and determined eight isoquinoline alkaloids, i. e. chelidonine, protopine,berberine, coptisine, tetrahydrocoptisine, 6- methoxydihydrochelerythrine, 6- methoxy-dihydrosanguinarine and dihydrosanguinarine. The HPTLC method developed used onedeveloping system on high performance silica gel plate (10×10 cm). After separationof these eight alkaloids, fluorescence derivatization was carried out in situ. The content wasdetermined by fluorescence scanning. TLC fluorescence derivatization, fluorescenceenhancement and fluorescence stability have been studied. Experiments showed that thismethod is simple, fast, highly sensitive and highly selective.