促红细胞生成素对实验性肾性贫血的作用

EFFECTS ERYTHROPOIETIN ON EXPERIMENTAL ANEMIA IN RATS WITH CHRONIC RENAL FAILURE

促红细胞生成素(erythropoietin,EPO)是由肾细胞分泌的一种糖蛋白激素。从人胚肾细胞中诱导,经生物化学方法分离、提纯得到此品。本试验用5/6肾切除的方法造成大鼠慢性肾衰性(CRF)贫血,研究不同剂量EPO对CRF贫血的作用。结果表明EPO有显著的促进红细胞生成,改善CRF贫血状态,使其接近或达到正常水平,最佳剂量为1000 U/kg,并可预防实验性贫血,对正常鼠未见明显作用。

Erythropoietin (EPO) is a glycoprotein hormone secreted by humankidney cells. Human EPO was induced from human embryo kidney cells, isolated and pu-rified from medium by biochemical method in our laboratory. The hypoproliferative ane-mia in chronic renal failure (CRF) has been assumed to be the result of decreased EPOproduction by the damaged kidney and of the shortening of the survival of erythrocytes. In this study, CRF anemia was formed 9 weeks atter the removal of five-sixths of therenal mass of rats. These anemic rats were divided into 6 groups: treated with differentdosages of EPO or physiological saline. The results indicate that EPO has apparent effects on anemia in rats with CRF. Itmay stimulate erythropoiesis and improve the anemia state of rats with CRF.Hemalotogical parameters (RBC, Hb, PLT, Ht and Rt )may be reverted to normal levels(P<0.001 ). The level of BUN and Cr were significantly decreased. The optimum doseof EPO was 1000 U/kg. All these results show that injection of EPO has therapeutic ef-fect on anemia in rats with CRF. EPO showed no effect on normal rats.