百部生药学研究——Ⅸ.中国百部属植物块根中总生物碱的测定与评估

PHARMACOGNOSTICAL STUDIES ON BAIBU RADIX STEMONAE AND ALLIED DRUGS——Ⅸ.DETERMINATION AND EVALUATION OF TOTAL ALKALOID CONTENT IN THE ROOTS OF CHINESE STEMONA SPP

百部类中药大多为百部科、百部属Stemona Lour.多种植物的块根,我国约6种,为常用中药,具润肺上气止咳、杀虫之功效。其活性成分之一为百部类生物碱,其个别种总生物碱的含量测定方法是酸碱滴定。尚未见其它方法的报道,本文探讨了用酸性染料比色方法测定中国百部属植物块根中总生物碱,较系统地比较总生物碱与百部属各种间、产地间。

An acid-dye colorimetric method was reported for the determination oftotal alkaloids in 53 samples of Baibu drugs from their growing destricts in 14 provincesand municipalities and its average recovery and its linear range were 96. 1% ( CV <4%)and 20～150μg respectively. The relationship between the total alkaloid content, geogra-phical origins and morphology were discussed. The results showed that: 1. the content of totalalkaloids of stemona was 0.26～3. 1 %; 2. that of Stemona sessilifolia was 0.26～2.17%with the highest content of the sample from Nanyang country in Henan Province; 3.that of S. japonica was 0.83～1.43%; 4. that of S. tuberosa was 0.53～3.1% with thehighest content of sample from Hengyang in Hunan Province; 5.that of S.parviflora was 0.22～0.74% with the highest content of sample from Qongzhong in Hainan Province;and 6.thatof more yellow, solider and stronger samples was higher than that of any other samples.Howerver, that of all bigger samples in shape was not higher than that of smaller ones.