硝普钠的光解分析法及其在透皮吸收研究中的应用

THE PHOTODEGRADATION-ASSAY METHOD OF SODIUM NITROP-RUSSIDE AND ITS APPLICATION TO STUDIES ON PERCUTANEOUS ABSORPTION

依据已报告的光解分析法原理,建立了定量分析硝普钠的方法。在光源照射下,硝普钠溶液在394nm处吸收度增加属非线性动力学,但对一定浓度溶液,吸收度增量对浓度呈直线关系;无论光照30min或60min,依据△A_(394)都能准确地定量。本文方法线性范围50～1000mg/L,日内变异系数<1.9％;日间变异系数<2.6％,回收率99.0～100.1％。共存的少量血清蛋白、十八醇、维生素B_(12)、丙二醇、氮酮以及间硝苯啶、尼群地平、维拉帕米等均不干扰测定。方法已成功地用于透皮吸收剂的研究。

A new method of quantitative analysis of sodium nitroprusside (SNP) was devel-oped according to the principle of a photodegradation analytical method that has been reported by theauthors previously. After irradiated of solution of SNP under fluorescent lamp, the increase in ab-sorbance at 394 nm belonged to nonlinear kinetics, but the absorbance increment with concentrationwas linear in certain concentration range of SNP. When irradiated for 30 min or 60 min,SNP in solu-tion can be quantitated accurately by ΔA_(394). The standard curve of this method was linear from 50 to1000 mg/L. The within-day and day-to-day precisions(RSD) were 1.9% and 2. 6% respectively,with recoveries of 99.0～100.1%. No interference from small amount of serum protein, stearyl al-cohol,propylene glycol, azone, m-nifedipine, nitrendipine and verapamil was observed. This methodhas been successfully applied to study percutaneous absorption.