白细胞介素-6在心脏成纤维细胞增殖及心肌纤维化过程中的作用

Role of interleukin-6 in cardiac fibroblasts proliferation and cardiac fibrosis

目的:观察白细胞介素-6(interleukin-6,IL-6)对心脏成纤维细胞(cardiacfibroblasts,CFs)增殖及p27蛋白表达的影响,探讨IL-6促CFs增殖的细胞分子生物学机制。方法:以培养的新生SD大鼠CFs为实验模型,采用四氮唑盐比色法检测细胞增殖,流式细胞分析仪技术测定细胞周期及p27蛋白表达的阳性率。结果:①1×107U/LIL-6作用48h,四氮唑盐比色法测定的CFs的A值为0.38±0.01,明显高于基础状态组(0.32±0.01),差异有显著性意义(t=-15.983,P=0.000)。②IL-6作用组CFs的S期细胞百分率(13.00±3.53)%和细胞增殖指数(PI)(29.43±1.94)%均高于基础状态组犤(7.45±0.97)%和(26.03±0.96)%犦,并有差异有显著性意义(t=-3.036,P=0.023;t=-3.142,P=0.020),而G0/G1期细胞百分率(70.58±1.94)%低于基础状态组(73.98±0.98)%,差异显著(t=3.142,P=0.020)。③IL-6作用组CFs的p27蛋白表达阳性率为(72.60±2.47)%,明显低于基础状态组(78.45±1.91)%,差异有显著性意义(t=3.753,P=0.009)。结论:IL-6能够诱导CFs增殖,其机制可能与p27蛋白表达水平下调有关。

AIM:To observe the effect of interleukin 6(IL 6) on proliferation and p27 expression in cardiac fibroblasts(CFs), and to explore the mechanism of IL 6 in CFs proliferation and cardiac fibrosis. METHODS: Neonatal SD rats CFs were cultured as a model.Cell proliferation was evaluated with MTT assay, and cell cycle distribution and positive rate of p27 expression were determined with flow cytometry. RESULTS:① The A value of CFs measured with MTT assay in IL 6(1× 107 U/L, 48h) group(0.38± 0.01)was significantly higher than that in the basic condition group(0.32± 0.01)(t= - 15.983,P=0.000);② The percentage of CFs in S stage[13.00± 3.53)% ] and proliferation index (PI) [29.43± 1.94)% ]in the IL 6 group were significantly higher than those in the basic condition group[(7.45± 0.97)% and (26.03± 0.96)% ](t=- 3.036,P=0.023;t=- 3.142,P=0.020),while the percentage of G0/G1 stage cells [(70.58± 1.94)% ] was obviously lower than that in the basic condition group[(73.98± 0.98)% ](t=3.142,P=0.020);③ Positive rate of p27 expression in the IL 6 group [(72.60± 2.47)% ] was significantly lower than that in the basic condition group[(78.45± 1.91)% ](t=3.753,P=0.009). CONCLUSION:IL 6 can induce CFs proliferation, and its mechanism may be related to the downregulation of p27 expression.