组织工程血管种子细胞的培养

Culture of seed cells in tissue engineering vessel

目的:为构建组织工程血管提供种子细胞。方法:全麻下取兔的主动脉,分别采用酶消化法和组织块贴壁法培养血管内皮细胞和平滑肌细胞,并传代、免疫组化鉴定及倒置显微镜下形态学观察。结果:成功地培养出血管内皮细胞和平滑肌细胞并传代。倒置显微镜下血管内皮细胞呈“鹅卵石”形态,平滑肌细胞呈典型的“峰-谷”状。免疫组化鉴定内皮细胞Ⅷ因子(+),平滑肌细胞α-肌动蛋白(+)。结论:所培养的血管内皮细胞和平滑肌细胞为研究组织工程血管提供了细胞来源。

AIM:To provide seed cells for tissue engineering vessel research. METHODS:Aortas of rabbits were excised under general anesthesia,vascular endothelial cells and smooth muscle cells were cultured respectively by enzyme digestion and tissue explantation method,subcultured and identified by morphological characteristics under phase contrast microscopy and immunohistochemical staining. RESULTS:Both of two kinds of cells were cultured and subcultured successfully.Vascular endothelial cells showed cobble appearance enchased arrange and smooth muscle cells“ hill and vale” appearance. Immunohistochemical staining results showed that endothelial cells were Ⅷ factor positive and smooth muscle cells α actin positive. CONCLUSION:Vascular endothelial cells and smooth muscle cells are important cell sources for tissue engineering vessel.