极低密度脂蛋白受体在糖尿病患者脂肪组织中的分布和表达(英文)

Distribution and expression of very low density lipoprotein receptor in adipose tissues of diabetics

目的:观察极低密度脂蛋白受体(VLDL-R)的两种亚型在糖尿病患者脂肪组织中的分布和表达,以探讨其变化及意义。方法:选择10例正常人及9例糖尿病患者,手术中采集其腹壁及肠脂垂脂肪组织,从脂肪组织提取的mRNA用半定量-PCR方法扩增。结果:①正常腹壁及肠脂垂脂肪组织VLDLⅠ,Ⅱ型受体均有表达,两部位各亚型受体比较,差异无显著性意义(腹壁t=-0.454,P=0.6499;肠脂垂t=0.870,P=0.3843)。②糖尿病患者腹壁及肠脂垂脂肪组织中,Ⅰ型受体的分布和表达量与正常相比无明显变化,差异无显著性意义(腹壁t=-0.082,P=0.9349;肠脂垂t=-0.327,P=0.7439)。但Ⅱ型受体表达明显减少甚至丢失,与正常相比差异有显著性意义(腹壁t=3.576,P=0.0003;肠脂垂t=2.597,P=0.0094)。结论:糖尿病代谢紊乱影响VLDL-R表达及亚型分布。

AIM:To observe the distribution and expression of two subtypes of very low density lipoprotein receptor(VLDL R) in diabetic adipose tissues to investigate their changes and significance. METHODS:Adipose tissues of the abdominal wall and epiploic appendages were collected from 10 normal persons and 9 diabetics during operation.The mRNA extracted from the adipose tissues was amplified by semi quantitative polymerase chain reaction(PCR). RESULTS:① There was expression of both Type I,II VLDL R in the adipose tissues of the abdominal wall and epiploic appendages of normal persons,but there was no obvious statistical difference between the two subtypes in receptor expressions in both regions(for abdominal wall:t=- 0.454, P=0.6499; for epiploic appendages,t=0.870, P=0.3843).② Compared with the normal ones,the distribution and expression of Type I receptor in the adipose tissues of the abdominal wall and epiploic appendageses of the diabetics showed no marked statistical significance(For abdominal wall:t=- 0.082, P=0.9349; for epiploic appendages,t=- 0.327,P=0.7439). However,the expression of diabetic Type II receptor decreased obviously, even disappeared,and there was obvious difference compared with the normal persons(For abdominal wall,t=3.576, P=0.0003; epiploic appendages, t=2.597, P=0.0094). CONCLUSION:Diabetic metabolic disorder affects VLDL R expression and its subtype distribution.