热矿泥浴对大鼠佐剂性关节炎的作用及机制(英文)

Therapeutic effect and mechanism of thermal mineral mud-bath against adjuvant-induced arthritis in rats

目的:观察热矿泥浴对大鼠佐剂性关节炎(AIA)的治疗效果及作用机制。方法:Wistar大鼠按AIA造模后,随机分为3组,分别给予热矿泥浴、普通黄泥浴共19d,空白对照组则不予处理。连续观察关节炎症积分,第30天取标本,检测血清肿瘤坏死因子(TNF-α)含量,关节的组织病理学改变,原位杂交法观察基质金属蛋白酶-1(MMP-1)和细胞间黏附分子(ICAM-1)mRNA在关节滑膜和软骨中的表达。结果:热矿泥浴能显著降低AIA的关节炎症程度,包括炎症积分,以及组织切片的滑膜细胞增生和软骨侵袭。热矿泥浴还能显著降低血液中的TNF-α含量,抑制滑膜和软骨细胞的MMP-1mRNA以及软骨细胞ICAM-1mRNA的表达。普通黄泥浴也能抑制软骨细胞的ICAM-1mRNA的表达。结论:热矿泥浴能显著抑制大鼠AIA的关节炎症,其机制与抑制TNF-α的产生以及MMP-1和ICAM-1在滑膜和软骨中的表达有关,且这种作用不单纯是泥浴的物理作用,可能主要是热矿泥浴的化学和生物学作用所致。

AIM:To observe the therapeutic effect and mechanism of thermal mineral mud bath(TMM) against adjuvant induced arthritis(AIA) in rats. METHODS:Wistar rats with AIA were divided into three group randomly, treated with TMM or loess mud bath(LM) for 19 days,respectively.The control group was treated with nothing.The arthritic scores were observed constantly until the 30th day,then the specimen of blood and joints was obtained.The serum tumor necrosis factor α (TNF α )were measured by ELISA, the histopathology of the inflamed ankle were observed,and the expression of matrix metalloproteinase 1(MMP 1) mRNA and intercellular adhesion molecule 1(ICAM 1) mRNA in the synovium and cartilage were detected using in situ hybridization. RESULTS:TMM significantly decreased the severity of arthritis,including arthritic scores,synovium cells proliferation and cartilage erosion in histopathology.Besides,TMM clearly deduced the content of serum TNF α and suppressed the expressions of MMP 1 mRNA in synovium cells and chondrocytes as well as ICAM 1 mRNA in chondrocytes.Meanwhile,LM also suppressed the expression of ICAM 1 mRNA in chondrocytes. CONCLUSION:TMM significantly inhibited adjuvant induced arthritis in rats, with suppression of serum TNF α production and the MMP 1 and ICAM 1 mRNA expression in synovium and cartilage. This therapeutic effect attributed to the colligation of physical chemical and biological effects from the thermal mineral mud bath.