泽泻醇提取物对高血糖小鼠血液生化指标及胰岛素的影响(英文)

Effects of rhizome alismatis extract on blood biochemical indices and insulin in hyperglycemic mice

目的:探讨泽泻醇提取物(rhizomaalismaticextract,RAE)对正常动物及糖尿病动物的血糖、血脂、胰淀粉酶及胰岛素的影响,为泽泻用于治疗糖尿病及康复措施的介入提供理论依据。方法:选择雄性昆明种小鼠29只,按随机抽签法分为4组:生理盐水组、格列齐特组、RAE10g/kg组、RAE20g/kg组。给小鼠尾静脉注射四氧嘧啶90mg/kg,造成高血糖动物模型。用自动生化分析仪测定血糖、血脂和胰淀粉酶。放免法测定血清胰岛素。光镜下观察胰腺和胰岛的组织学变化。结果:RAE一次灌胃20g/kg,可使正常小鼠血糖明显降低犤对照组(7.6±1.1)mmol/L;RAE20.0g/kg组(5.6±1.5)mmol/L,(t=2.329,P<0.01)犦。重复治疗7d,RAE10,20g/kg使四氧嘧啶小鼠血糖降低犤对照组,RAE10g/kg组,RAE20g/kg组血糖值分别为(21.9±2.8),(15.7±5.7),(9.3±3.9)mmol/L(t=2.329,P<0.05,t=3.118,P<0.01)犦。光镜下观察:四氧嘧啶小鼠胰岛数量减少,形态也明显缩小,而用RAE治疗动物的胰岛组织学未见明显变化。RAE20g/kg还使正常小鼠犤对照组,RAE20g/kg组的胰岛素水平分别为(16.4±4.0),(24.6±8.1)μU/L犦及四氧嘧啶小鼠的胰岛素水平犤对照组,RAE20g/kg组的胰岛素水平分别为(13.5±4.3),(20.1±9.1)μU/L犦增加。结论:RAE具有明显的降血糖和降血脂作用。

AIM:To explore the effects of rhizome alismatis extract(RAE) on blood glucose,blood lipid,pancreatic amylase and insulin in normal and diabetic mice so as to provide scientific basis for the cure of diabetes with rhizome alismatis and intervention of rehabilitation measures. METHODS:Twenty nine male mice from Kunming were randomly divided into 4 groups:saline group, gliclazide group,RAE 10 g/kg group and RAE 20 g/kg group.Alloxan(90 mg/kg) was injected into the caudal veins of the mice to establish hyperglycemia models.Blood glucose,serum lipid and pancreatic amylase were determined using automatic biochemistry analysor, and serum insulin was determined with radioimmunoassay.The pathological changes of pancreas and islets were observed under light microscope with HE staining and paraffin section method. RESULTS:RAE,20 g/kg ig,reduced the blood glucose in normal mice significantly[control group(7.6± 1.1) mmol/L;RAE 20 g/kg group (5.6± 1.5) mmol/L,P< 0.05,t=2.329].RAE,10 and 20 g/kg ig, also decreased blood glucose after 7 day repeated administration in alloxan induced hyperglycemic mice[control group( 21.9± 2.8) mmol/L;RAE 10 g/kg group(15.7± 5.7) mmol/L,P< 0.05,t=2.817;RAE 20 g/kg group( 9.3± 3.9) mmol/L,( t=2.329,P< 0.05,t=3.118,P< 0.01) ].Pathological examination: both the number and the size of islet tissues were reduced in alloxan induced diabetic mice,while there were no significant changes in those treated with RAE.RAE(20 g/kg) also increased the release of insulin in normal mice[control group(16.4± 4.0) μ U/L, RAE 20 g/kg group(24.6± 8.1) μ U/L]and alloxan mice[control group (13.5± 4.3) μ U/L,RAE 20 g/kg group(20.1± 9.1) μ U/L]. CONCLUSION:RAE possesses potent effects of lowering blood glucose and serum lipid,and protecting islets from injury.Its anti hyperglycemic mechanism may be associated with promoting release of insulin from beta cells in islets.