大便隐血单克隆抗体检查方法的优化

Optimization of the monoclonal antibody assay used for detecting occult blood in the stool

目的 了解增强液 (改进稀释液 )是否优于 DH2 O(原法稀释液 ) ,从而提高实验的灵敏度。方法 通过改进反应介质 ,选择抗原抗体反应最适浓度。患者样本采用两种方法进行随机对比分析。结果 经 STAT医学统计软件包处理 ,其χ2 =6 7.2 8,P<0 .0 5 ,显示两法的检验效能不一致。结论 采用增强液做抗原抗体反应介质检测大便隐血优于用 DH2

Objective To understand the advantage of strengthened liquid(improved diluent liquid)and raise the test sensitivity.Methods We improved the element of reactivity,chosed the most suitable concentration of the reaction and analyzed the results of the two kinds of methods randomly.Results The results was calculated with statistic software package(χ 2=67.28, P <0.05).The efficiency was different between the two kinds of methods.Conclusion The improved diluent liquid is superior to original liquid in detection of stool occult blood.