尿路清的抗炎抑菌实验

Experimental study of niaolvqing's anti-inflammation and inhibition on bacteria

目的 观察尿路清的抗炎抑菌实验疗效。方法 利用二甲苯致炎 ;大鼠肩胛区注入松节油致炎 ;大鼠右后足跖皮下注射角叉菜胶致炎 ;并利用炎性肿胀足 ,剪碎离心 ,提取上清液测定PGE2 的含量 ,体外抗菌实验测定MIC、MBC。体内抗菌实验分别观察尿路清对金葡菌、大肠杆菌抑菌作用。结果 尿路清高中低组均可明显抑制二甲苯诱导的大鼠耳肿胀 ;明显抑制松节油导致的气囊肉牙增生 ;对角叉菜胶诱导的大鼠足肿胀有显著疗效。尿路清高剂量组可显著降低肿胀足组织中PGE2 的含量。对大肠杆菌、痢疾杆菌、变形杆菌、金黄色球菌、表皮球菌、金黄色球菌 (ATCC)、大肠杆菌 (ATCC)均有不同程度的抑菌和杀菌作用。尿路清 ( 16 g/kg)对金葡菌、大肠杆菌感染的小鼠有一定的保护作用。

Objective To observe the effect of anti inflammation and inhibition on bacteria of niaolvqing.Methods Inflammation was induced by dimethylbenzene,turpentine oil and carrageenan at differential locus of mice or SD rats.The inflammatory foots of SD rats were broken and centrifugated,then superior liquor was extracted for determinating PGE 2.The effect of niaolvqing's inhibition on bacteria in vitro was measured by MIC and MBC.The effect of niaolvqing's inhibition on staphylococcus and bacillus coli in vivo also was observed.Results Niaolvqing at differential concentrations all could inhibit the mice ear's swelling induced by dimethylbenszene and the hyperplasia of balloon granulation induced by turpentine oil,niaolvqing also could inhibit significantly the rat foot's swelling induced by carrageenan,niaolvqing at high concentration reduced markedly the level of PGE 2 of rat foot tissue and had the inhibition on staphylococcus,bacillus coli and so on to vary extent,which(16g/kg) had some protection on mice infected by staphylococcus and bacillus coli.Conclusion Niaolvqing have good effect of anti inflammation and inhibition on bacteria.