大鼠中脑黑质神经元的增龄性变化

Changes of Neurons in Substantia Nigra of Middle Brain of Rats Associated with the Increasing Age

目的 :探讨大鼠中脑黑质神经元的增龄性变化规律。方法 :SD大鼠 32只 ,雌雄各半 ,分为幼年组 (1～ 2月龄 )、青年组 (4～ 5月龄 )、中年组 (1 1～ 1 2月龄 )和老年组 (≥ 2 4月龄 ) 4个年龄组 ,常规石蜡切片 ,取中脑黑质连续冠状切片 ,进行焦油紫染色和TUNEL法细胞凋亡染色 ,用图像分析仪对焦油紫染色细胞及TUNEL法染色阳性神经元 (凋亡细胞 )进行计数。结果 :随着年龄增长 ,大鼠中脑黑质神经元数量减少 (P <0 0 5 ) ,黑质神经元凋亡增加 (P <0 0 5 )。结论 :中脑黑质神经元随年龄增长而出现的衰老性变化 ,可能是黑质病变的原因之一。

Objective: To study the regularities of changes of neurons in substantia nigra of middle brain of rats with increasing age. Methods: 32 SD rats were used and divided into four groups: 1～2 months, 4～5 months, 11～12 months and above 24 months. Routine paraffin sections were made for crystal violet staining, terminal transferase and biotin dUTP nick end labeling (TUNEL) staining. The neurons and the TUNEL positive neurons (apoptotic cells) in the substantia nigra were analyzed with morphoretric technique. Results: The numbers of neurons in substantia nigra of middle brain decreased with increasing age ( P<0 05 ) and the number of apoptotic cells increased along with the age increasing ( P<0 05 ). Conclusion: The increasing decrepit change of neurons in substantia nigra with age increasing might have important implication for the pathogenesis of substantia nigra disease in this region.[