生防菌株2P24与CPF-10的鉴定及其生防相关性状的初步分析

Identification and characterization of biocontrol bacterial strain 2P24 and CPF-10

从山东省小麦根围土壤中分离到具有生防活性的细菌菌株2P24、CPF-10。通过对其16S rDNA序列同源性分析及生理生化测定鉴定为荧光假单胞菌(Pseudomonas fluorescens),其中菌株2P24为生物型I,CPF-10为生物型V。对这2株细菌的生防相关性状分析表明,二者均可产生多种抗菌物质,如2,4-二乙酰基间苯三酚(2,4-DAPG)、氢氰酸(HCN)、嗜铁素、蛋白酶等。平板对峙测定表明2株细菌对番茄青枯菌(Rastonia solanacearum)、棉花立枯丝核菌(Rhizoctonia solani)、棉花枯萎菌(Fusarium oxysporum)具有明显的拮抗作用。温室生测表明2P24对番茄青枯病的防效达63.0％。CPF-10达62.4％,且持续稳定。

Bacterial strains 2P24 and CPF-10 are two biocontrol agents isolated from wheat rhizosphere in Shandong Province. These two strains were classified into the genus of Pseudomonas by analyzing the 16S rDNA sequence. Further detection of physiological and biochemical characteristics revealed that both strains belonged to P. fluorescents The strain 2P24 fell into biovar I and CPF-10 fell into biovar V. Both 2P24 and CPF-10 produced several antifungal compounds, such as antibiotic 2, 4-di-acetylphloroglucinol (2,4-DAPG), hydrogen cyanide (HCN), siderophore (s) and extracellular pro-teinase, and showed inhibitory to Kastonia solanacearum, Fusarium oxysporum and Rhizoctonia solani in the dual-culture test in plates. In greenhouse, strain 2P24 and CPF-10 showed 63. 0% and 62. 4 % efficiency to control tomato bacterial wilt, respectively.