千金藤素抗单纯疱疹病毒Ⅰ型(HSV-1)初探

Study on the Inhibitory Effect of Cepharanthine on Herpes Simplex Type-1 Virus(HSV-1) in Vitro

目的 :体外测定千金藤素抗单纯疱疹病毒Ⅰ型的作用 ,为筛选新型抗病毒药物提供参考依据。方法 :用不同稀释度的千金藤素抑制单纯疱疹病毒Ⅰ型对Vero细胞的感染作用 ,4 8h后观察细胞病变 (CPE)效应。结果 :千金藤素能明显抑制HSV 1对Vero细胞的致病变作用 ,使细胞存活率升高。结论 :千金藤素有较显著的抗HSV 1的作用 ,且抗病毒作用是多方面的 ,是一种具有潜在开发前景的药物。

Objective: To assay the inhibitory effects of cepharanthine on HSV-1 in vitro. Mehtods: Vero cells were infected by HSV-1 and cultured with serial dilutions of cepharanthine. The inhibitory effects of cepharanthine were evaluated with cytopathogenic effects (CPE). Results: The CPE of cells infected by HSV-1 were inhibited significantly, resulting in higher cell survival rates. Conclusion: The results showed that cepharanthine had obvious HSV-1 inhibitory efficacy. Therefore, it may become a potential candidate in drug development and deserves further research on animal experiment and antiviral mechanisms.