定量RT-PCR检测肝细胞P450酶基因与苦参的关系

Investigation on the relationship between liver microsome cytochrome P450 activity ( mRNA) and kang-ke injection solution

目的 明确肝脏微粒体中细胞色素P450酶活性与苦参中对抗CVB病毒的有效成分抗柯注射液药物剂量及用药时间的关系。方法 用定量RT-PCR法,在Begale犬长毒试验的不同时期(45、90、135 d),用不同剂量抗柯注射液(7.5、15、30 mg/kg)检测肝脏微粒体中细胞色素P450酶基因(β-actin/P450)的特异mRNA。结果 P450酶基因(β-actin/P450)特异信使核糖核酸mRNA随所用抗柯注射液剂量及用药时间而增加(P<0.01)。结论 P450的比值随药物剂量及时间延长而上升,可视为机体抵抗中毒而作出的一种适应性反应,可用于有关药物的毒理研究。

Objective To understand the relationship between the cytochrome P450 enzyme activity from liver mi-crosome and the active substance of 'kushen' against CVB. Methods Use quantitative RT-PCR method to detect P450 enzyme activity ( mRNA) from the liver microsome of Beagle dog during long term toxicity investigation using different doses of kang-ke injection solution (7.5、15、30 mg/kg) and at different times (45, 90, 135 d). Results The increase of P450 (mRNA) was proportional to the increase of kang-ke injection fluid dosage and its duration. Conclusion The P450 activity was increased in proportion to the kang-ke injection fluid dosage and duration. This would be considered as the adaptive reaction against intoxication.