移植物与受体的不同T细胞比例对GVHD的影响

Effect of different T cell ratios between graft and receptor on GVHD during transplantation

目的 建立大鼠异基因骨髓移植模型,探讨移植时移植物内T细胞和经预处理后受体残存T细胞的不同比例对移植物抗宿主病(GVHD)的严重程度的影响。方法 受体SD大鼠接受TBI+CTX预处理,分别接1:1、2:1、4:1的移植物与受体T细胞比例回输供体Wistar大鼠骨髓细胞,观察受体生存期、临床及病理GVHD评分。结果 实验组大鼠按移植物与受体T细胞比例为1:1和2:1移植时生存期、临床及病理GVHD评分与4:1时有显著性差异(P<0.05)。结论 移植时当移植物内T细胞和经预处理后受体残存T细胞比例为1:1、2:1时GVHD程度较轻,4:1时GVHD明显加重。

Objective In this study we setup rat allo- stem cell transplantation model to discuss the effect of different T cell ratio between donor to receptor at the time of transplantation on graft-versus-host disease ( GVHD ). Methods SD rats were selected as receptor and receive TBI + CTX as pretreatment regimen. Infuse the receptors with different ratio of donor's bone marrow cell. Observe the life-span, clinical and pathological GVHD scores. Results When the graft-receptor T cell ratios were 1: 1 and 2 : 1 ,the life-span,clinical and pathological GVHD score is significantly different from the 4: 1 ratio. Conclusion When the ratio between graft and receptor is 1: 1 or 2: 1 , the GVHD is mild. When this ratio reaches 4: 1 , the GVHD becomes more severe.