摘要
为得到具有较强抗虫性的新的芥菜种质资源,用农杆菌介导将豇豆胰蛋白酶抑制剂(CpTI)基因导入芥菜,获得了Kan抗性植株。经PCR扩增、PCR Southernblot和Northernblot分析,转化再生植株大部分呈阳性,而非转化的再生植株均为阴性,证明CpTI基因已存在于芥菜基因组中。在室内进行了喂虫试验,结果表明转基因芥菜抗虫性明显高于对照,转基因植株之间存在抗虫性差异。
Explants of mustard were transformed with Agrobacterium tumefaciens (LBA4404) to get new source of insect-resistant mustards.The putative transformation regeneration plants were assayed by PCR,PCR-Southern blot and Northern blot analysis.By these analyses,the target bands were observed,the integration of the Cowpea trypsin inhibitor(CpTI)gene into mustard genome DNA was confirmed.The result of insect-resistance showed that the transgenic plants were more resistant than non-transgenic plants.
出处
《西南农业学报》
CSCD
2004年第1期74-77,共4页
Southwest China Journal of Agricultural Sciences
关键词
豇豆胰蛋白酶抑制剂基因
芥菜
转化
抗虫
cowpea trypsin inhibitor gene
Brassica juncea
transformation
insect-resistance
