萝卜自交不亲和基因的利用(英文)

Uses of self-incompatibility genes in Chinese radish

利用荧光显微镜技术和种子结实分析方法对7个中国型萝卜(RaphanussativusL.var.longipinnatusBailey)自交系进行研究其自交不亲和水平。试验结果表明多数自交系为自交不亲和。采取完全杂交组合方法进行筛选。从品种比较和结实情况看,杂一代27 1 3×18 1,27 1 3×18 2,27 1 3×18 3和27 1 3×18 6以及部分77 2×18 11和77 4×18 7有较好的园艺性状,如整齐度、叶色深绿无毛、肉质根呈圆筒状、根皮光滑、根质紧实、根重360～400g,这些性状符合市场要求。前期试验结果表明,这些杂一代的根产量都明显高于普通品种,但还有待在农户的地里做进一步的试验。

Seven inbred lines of Chinese radish (Raphanus sativus L. var.longipinnatus Bailey) were investigated for their self-incompatibility levels using fluorescent microscope technique and seed setting analysis. Results showed that most of the inbred lines were self-incompatible. They were crossed in all combinations to select for the best hybrid variety. From a varietal trial and an ability to set seeds, it was found that hybrids 27-1-3×18-1, 27-1-3×18-2, 27-1-3×18-3 and 27-1-3×18-6 and some of hybrids 77-2×18-11 and 77-4×18-7 had good horticultural characteristics such as uniformity, dark green leaf without hair, cylindrical shape, smooth skin, firm texture of root and root weight of about 360-400 g. These characteristics are market-acceptable. Root yield of these hybrids was shown in previous experiments that it was a lot higher than market varieties. The hybrid varieties should be further tested in farmer's field.