四川葡萄病毒病田间普查及血清学鉴定

Field investigation and serological detection of grapevine viruses in Sichuan province

对四川葡萄主产区病毒病的发生和危害状况进行田间普查,并采集了48份葡萄枝条样本进行病毒病的血清学检测和鉴定。经双抗体和三抗体夹心酶联免疫法测试后,从采自四川的这批样本中分别检测出了葡萄茎沟病毒(GVA)、葡萄栓皮病毒(GVB)、葡萄扇叶病毒(GFLV)、葡萄斑点病毒(GFkV)、葡萄卷叶病毒-2(GLRaV 2)和葡萄卷叶病毒-3((GLRaV 3)6种危险性的病毒(株系),它们的检出率分别为14 6%、8 3%、10 4%、27 1%、14 6%和20 8%,另外两种靶标病毒(株系)葡萄卷叶病毒-1(GLRaV 1)和南芥菜花叶病毒(ArMV)则未被发现。在四川省内一些老龄葡萄园内,以及部分引进的葡萄新品种上,病毒病的发生和流行极为普遍,并给葡萄的正常生长和生产造成了严重危害。

In this paper,a field survey on grapevine viruses was carried out in Sichuan province. During the course of investigation, 48 stem samples were collected and then sent to Italy for evaluation of their sanitary status by Das-or Tas-ELISA. The results showed that six dangerous grapevine viruses (or strains) were detected from these samples, including GVA, GVB, GFLV, GFkV, GLRaV-2 and GLRaV-3 with infective rates of 14.6%,8.3%,10.4%,27.1%,14.6% and 20.8%, respectively. Other two target viruses of GLRaV-1 and ArMV were not present in the samples. In some old grapevine orchards and freshly introduced varieties, dangerous grapevine viruses were widely spread and caused serious damage to growth or production of grapevine plants.