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膜技术分离七叶参皂甙研究 被引量:14

Study on the Separation of Saponin of Gynostemma Pentaphyllum Makino by Membrane Technology
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摘要 以七叶参为材料,研究了微滤澄清、超滤除大分子、纳滤分离、纳滤浓缩等膜技术在分离七叶参皂甙的应用效果。结果表明,采用0.2μm微滤膜在40℃条件下微滤经粗滤后的七叶参浸提液,且在微滤滤液达到浸提液重的80%时加入与原料等重的透析水透析残渣可作为微滤澄清的技术参数;选用截留分子量为10000Da的超滤膜能有效去除色素等大分子物质;利用截留分子量为2500Da或3500Da的纳滤膜分离七叶参皂甙,可制得纯度为42%以上的七叶参皂甙产品,且3500Da纳滤膜比2500Da纳滤膜的得率要高,但干粉中皂甙纯度略低;选用500D的纳滤膜在32℃左右、操作压力为1.8MPa的条件下浓缩七叶参皂甙不仅浓缩效率高,而且可进一步提高产品纯度。 By using the Gynostemma Pentaphyllum Makino as material,the experiment was carried out in order to study theapplication effect on the separation of saponin of Gynostemma Pentaphyllum Makino by the membrane technology of micro-filtration to clarify,ultra-filtration to get rid of big molecule substance,nanofiltration to separate or concentrate.The resultsshowed that 0.2μm micro-filtration membrane could clarify the extracted liquid efficiently at 40℃ when added the same weightdialysis water as the extracted material when the weight of filtrated liquid reached 80% the weight of extracted liquid;The ultra-filtration membrane whose holding up molecule was 10000Da could get rid of big molecule substance ;The saponin products ofGynostemma Pentaphyllum Makino whose content of saponin was higer than 42% could be obtained by choosing the 2500Dor 3500D nanofiltration membrane,furthermore,3500D nanofiltration membrane had a little higher rate of preparation than thatof 2500D nanofiltration membrane,but the purity of saponin in the dried products had the reverse result;At last,concentratingthe saponin of Gynostemma Pentaphyllum Makino by choosing the 500D nanofiltration membrane on the condition of 32℃and 1.8MPa not only could have higher concentration rate ,but also could enhance the purity of products.
出处 《食品科学》 EI CAS CSCD 北大核心 2004年第2期52-56,共5页 Food Science
基金 国家计委十五高新技术产业化重大专项资助(计高技[2002]2284号)
关键词 七叶参 皂甙 膜技术 分离 微滤 超滤 纳滤 提取 药用 saponin of gynostemma pentaphyllum makino micro-filtration ultra-filtration nanofiltration
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