阿勒泰山林区莱姆病染毒实验小鼠肝肾肺脑组织的病理形态学观察

Studies on the Pathologic Features of Lyme Dissease of mice in Arlartai Area of Xinjiang Province

目的 :为了深入了解新疆阿勒泰山林区莱姆病伯氏疏螺旋体致病之病理特征。方法 :采用从当地林区媒介分离到的螺旋体接种于 2 0天的实验小白鼠腹腔和皮下 ,于接种后 3 0天、60天、90天、12 0天分别处死实验小鼠 ,取肝、肾、肺、脑脏器做病理切片 ,光镜下观察。结果 :对照鼠 10张 (只 ) ,其肝、肾、肺脑组织切片均未见有异常。光镜下 10张 (只 )实验鼠肝、肾、肺、脑组织有异常 ,且其肝组织切片示肝组织炎症损害 ,汇管区以淋巴细胞浸润为主 ;肾组织切片示以肾皮质的慢性炎症损害为主 ;肺组织切片示肺组织以间质性肺炎改变 ;脑组织切片示脑组织脑膜充血反应 ,神经细胞退变 ,胶质细胞增生 ,淋巴细胞浸润 ,结节形成。结论 :小白鼠感染伯氏疏螺体后可致多脏器损害 ,临床上对于上述症状体征的病畜。

Objective :To investigate the pathologic features of Lyme Disease (LD) of mice in the south foot of west and middle Arlartai mountains and forest zones.Methods The change of Surgical specimens in cluding liver,renal,lung,brain on day 30,60,90 and 120after Borrelia buegdorferi (Bb) inoculation in mice were observed by the light microscopy. Results: Each pathologic observation consisted of 10 LD mice and 10 control mice. Histopathologically,liver tissue was demonstrated architectural destruction like hepatitis ,lymphocyte was the predominant inflammatory cells in portal area;renal tissue was observed by chonic inflammation ,especially in cortex renalis;lung tissue was destructed by intersticial lung inflammmation;The pathologic changes in brain were congestive meninges action,neurocyte degeneration,gliocytic proliferation,limphocytic infiltration and nodular hyperplasia. Conclusions: The pathologic changes of multiple system organs were observed in LD mice. The evaluation of pathologic features are useful and pratical for diagnostic and defferential purpose in lyme disease of human and animals.