乙型肝炎患者血清自身抗体检测的研究

Study on Autoantibodies in Patients with HBV

目的 检测乙型肝炎患者血清自身抗体并分析其临床意义。方法 以 HEP- 2细胞、鼠胃和鼠肾组织为抗原 ,采用间接免疫荧光法对 1 1 7例乙型肝炎患者血清及 30例正常人血清作抗核抗体、抗平滑肌抗体和抗线粒体抗体检测。结果 乙型肝炎患者自身抗体总阳性率为 1 8.8% ,高于正常对照组 ,差异有显著性 (χ2 =4.1 9,P<0 .0 5 )。自身抗体以低滴度为主 ,多见于抗平滑肌抗体和抗核抗体。结论 乙型肝炎患者存在多种自身抗体 ,观察其自身抗体的滴度与类型对乙型肝炎患者的治疗有一定的参考价值。

Objective To study the clinical significance of determining serum autoantibodies in HBV patients.Methods Hep-2 cells,rat ren tissue and rat stomach tissue as substrates for ANA,AMA and ASMA were detected by immunofluorescence assay in the serum of 117 HBV patients and 30 normal subjects.Results The positive rate of autoantibodies in HBV patiensts was 18.80%,and there was significance different between the groups of HBV and normal (P<0.05).Low titer of autoantibodies were presented in HBV patients,and most of them were AMA and ASMA.Conclusion HBV patients have many kinds of autoantibodies.The observation of autoantibodies titer and type are helpful for treatment of HBV patients.