抗线粒体-M_2抗体在原发性胆汁性肝硬化的意义

The Significance of Antimitochondria Antibody-M_2 Subtype (AMA-M_2) to Primary Biliary Cirrhosis(PBC)

目的 探讨抗线粒体抗体 - M2 亚型 ( AMM- M2 )对原发性胆汁性肝硬化 ( PBC)的意义。方法 应用酶免疫斑点法 ( DIBA)检测抗 AMA- M2 抗体 1 63例 ,M2 抗原包被区出现深蓝或紫色条带为阳性 ,无颜色为阴性。结果 PBC患者 39例 ,AMA- M2 抗体 38例阳性 ,阳性率 97.4% ;自身免疫性肝炎患者 1 6例 ,AMA- M2 抗体 1例阳性 ,阳性率为 6.3% ;慢性乙型肝炎、系统性红斑狼疮和正常对照组均阴性。 PBC患者 AMA- M2 抗体阳性率明显高于其它组 ( P<0 .0 1 )。该法灵敏度为 97.4% ,特异性为 98.9%。结论 DI-BA检测 AMA- M2 抗体灵敏度高 ,特异性强 ,可作为 PBC重要的血清学指标 ,同时结合临床症状、生化检查、肝组织活检等提高 PBC的诊断率。

Objective To study diagnosis significance of antimitochondria antibody-M 2 subtype (AMA-M 2) to primary biliary cirrhosis(PBC).Methods Using DIBA to detect AMA-M 2 of 163 cases.It was positive when the coating area of M 2 antigen showed blue or purple strap and negative with no color.Results 39 cases were the patients with PBC,and 38 cases were the patients with positive AMA-M 2.The positive rate was 97.4%.16 cases were the patients with autoimmune hepatitis and only one case was the patient with positive AMA-M 2 .The positive rate was 6.3%.Chronic Hepatitis B group,systemic lupus erythematosus(SLE) group and normal matched contol group were all negative AMA-M 2.The positive rate of AMA-M 2 of PBC patients was much higher than that of other groups (P<0.01) .The susceptibility of this method was 97.4%.The specificity was 98.9%.Conclusion The method of detecting AMA-M 2 by DIBA has higher susceptibility and better specificity.So AMA-M 2 can be an important serology mark.Positive AMA-M 2 with clinical symptom,biochemistry examination,liver biopsy can enhance the diagnosis rate of PBC.