大鼠烫伤后血浆CGRP和NPY水平变化对肠粘膜屏障损害的影响

Effects of Plasma CGRP and NPY Level Changes on Intestinal Mucosal Barrier Injury after Scald in Rats

目的 :探讨大鼠烫伤后血浆CGRP和NPY变化及其意义。方法 :应用放射免疫分析测定烫伤后不同时间点血浆CGRP和NPY含量。结果 :大鼠烫伤后血浆CGRP逐步降低 ,1 2小时组最低 ,2 4小时组乃明显低于对照组 ;烫伤后血浆NPY逐步升高 ,1 2小时组最高 ,2 4小时组基本恢复正常 ;烫伤后血浆CGRP和NPY的变化呈负相关。结论 :烫伤后血浆CGRP降低 ,NPY升高 ,且呈负相关 ,CGRP。

Objective To investigate the significance of plasma CGRP and NPY levels changes immediately after scald in rats. Methods Thirty-two rat models of 30% TBSAⅢ°scald were prepared. Eight animals each were sacrificed at 3,6,12 and 24hrs; taking blood samples for determination of plasma CGRP, NPY levels and 5cm of ileum for pathologic study. As controls, eight animals without scald were treated in the same way. Results Plasma CGRP levels were decreased significantly after scald, reaching bottom value at 12hr and remained lower than those in controls at 24hr ( p <0 05). Plasma NPY concentrations were significantly elevated after scald, reaching peak value at 12hr and grudually decreased to normal at 24hr (vs controls, p >0 05). Plasma levels of CGRP were negatively correlated to plasma NPY levels ( p <0 01). Ileum mucosal injuries presented as edema, congestion with necrosis and slough of epithelium were most marked at 12hr. Conclusion Plasma CGRP and NPY levels changed significantly after scald and were mutually negatievely correlated. Post-scald intestinal mucosa barrier injuries were possibly related to the changes of levels of those vasoactive peptides.