肝硬化患者血浆二胺氧化酶及内毒素检测的临床意义

Detection and Clinical Significance of Plasma Diamine Oxidase and Endotoxin in Hepatocirrhosis Patients

目的 :探讨肝硬化患者血浆二胺氧化酶 (DAO)和内毒素水平的临床意义。方法 :以 5 0例肝硬化患者和3 0例健康体检者为试验组和对照组 ,用分光光度法检测其外周血中DAO和内毒素的活性。结果 :Child PughA、B、C 3组肝硬化患者DAO活性均升高 ,与对照组比较差异有显著性意义 (P <0 0 1) ;Child PughC级组DAO活性低于Child PughB级 ,两者差异有显著性意义 (P <0 0 1)。Child PughA级组内毒素活性与对照组比较 ,差异无显著性意义 (P >0 0 5 ) ,Child PughB、C级组与对照组比较 ,差异有显著性意义 (P <0 0 1)。 结论 :血浆DAO水平是肠粘膜损伤早期诊断的敏感指标 ,内毒素血症、肠粘膜屏障衰竭是肝硬化患者病情加重的重要因素 ,也是治疗的关键之一。

Objective: To evaluate the significance of changes in diamine oxidase(DAO) and endotoxin activity in hepatocirrhosis. Methods:50 hepatocirrhosis patients were selected as experimental group while 30 healthy people as control group. The concentration of DAO and endotoxin were detected in systemic circulation by spectrophotogrphy. Results:The concentration of DAO in experimental group was significantly higher, while that in Child-Pugh C was significant lower than that in child-Pugh B,compared with control group, endotoxin of experimental group had significant increase except Child-Pugh A. Conclusion:These data suggest that plasma DAO activiity may be a sensitive marker in early diagnose of gut failure, endotoxemia and the intestinal barrier function failure may be critical in hepatocirrhosis deterioration.