极端污染环境下草苷膦抗性菌株HTG7的筛选及其特性研究

SEPARATION AND CHARACTERIZATION OF GLYPHOSATE-TOLERANT BACTERIAL STRAIN HTG7 FROM EXTREMELY POLLUTED ENVIRONMENT

从被草苷膦极度污染的土壤中分离到一株极端抗草苷膦菌HTG7,经初步鉴定其为可变盐单胞菌 (Halomonasvarabilis) ,该菌株最高可以耐受 5 0 0mmol L左右的草苷膦浓度。电镜观察表明 ,5 5 0mmol L的草苷膦浓度下 ,细胞大量坏死。生理生化特性表明该菌最适pH为 7 0 ,并且在氯化钠浓度 0～ 1 0 %生长良好。

Bacterial strain HTG7 was isolated from extremely glyphosate-polluted soil. It was identified as Halomonas varabilis preliminarily . It could tolerate in 500 mmol/L glyphosate concentration. Micrograph of HTG7 demonstrated that almost no cells lived well in the 550 mmol/L glyphosate concentration. General characterization of strain HTG7 showed that the optimal pH was 7.0. It grew well in the NaCl concentration range from 0 to 10%.