红莲型杂交水稻红莲优6号及其亲本苗期与分蘖期根系基因表达差异分析

Differential Display on Gene Expression of Root System in Hybrid Rice Honglianyou 6 and Its Parental Lines at Seedling and Tillering Stages

运用mRNA差异显示技术 (DDRT PCR)对红莲型杂交稻组合红莲优 6号及其亲本 (粤泰A、扬稻 6号 )、保持系(粤泰B)苗期、分蘖期根系的基因表达状况进行了分析。结果显示 ,不同发育时期之间差异表达基因远少于同一时期之间杂种与亲本差异表达基因 ,苗期和分蘖期差异表达基因数量相近 ,但同一差异表达类型基因的数量有较大变化 ,分蘖期杂种表达单亲基因增多 ;互补表达基因可能对红莲优 6号杂种优势的形成起重要作用。MF1差异片段已通过Northern杂交验证 ;MF1片段克隆测序结果在GeneBank中进行同源序列查对 ,发现是新的cDNA序列。

The gene expression in root system for hybrid rice, Honglianyou 6, and its parental lines (Yuetai A, Yuetai B, Yangdao 6) at seedling and tillering stages was analyzed by DDRT PCR. Results revealed that differentially expressed genes between seedling stage and tillering stage were far less than those between hybrid and its parental lines at the same developmental stage, both differences in gene expression were detected; the number of the differentially expressed genes at tillering stage was close to that at seedling stage, while the gene number of the same differential expressed patterns was obviously different. The expressed genes in hybrid rice from one parent increased at tillering stage; heterosis in Honglianyou 6 probably owed to the complementary expression of gene. Blasting of MF1 after sequencing in GeneBank revealed that it was a new cDNA sequences. The fragment MF1 was confirmed through Northern blotting analysis.