稻瘟病菌nit突变体的诱导及其生物学性状

Recovery and Biological Properties of Nitrate Nonutilizing Mutants of Magnaporthe grisea

将 6个稻瘟病菌 (Magnaporthegrisea)菌株分别在含 6 0 g/L氯酸钾的MM培养基上培养 4 0d后 ,共得到 11个硝酸盐利用缺陷突变体 (Nitratenonutilizingmutants,简称nit突变体 ) ,频率为 1.4 2 %。比较了各nit突变体与亲本之间在无性和有性阶段的主要生物学性状 ,结果表明 ,所有的nit突变体均对氯酸钾产生了抗性。在YPSA平板上的生长速率、在TPSA平板上的产孢量和致病能力等重要的生物学性状都与亲本没有显著差异 ,交配型没有发生改变 ,但可交配菌株产子囊壳的能力与亲本有显著差异。因此 。

Eleven nitrate nonutilizing (nit) mutants were recovered from six isolates of Magnaporthe grisea cultured on MM media amended with 60 g/L potassium chlorate, with a frequency of 1.42 %. Some biological properties, such as growth rate, growth biomass, cultural characters, conidial production, sexual reproduction ability, and pathogenicity were compared between nit mutants and their parental isolates. Results showed that all the nit mutants were resistant to chlorate. Some important biological properties such as growth rate on YPSA, conidial production ability on TPSA, and pathogenicity, had no significant differences between nit mutants and their parental isolates. Mating type of the mutants hadn’t changed, but perithecia production ability of fertile isolates changed significantly as compared with that of their parental isolates. Therefore, the nit can be used as a genetic marker for study the genetics such as pathogenicity, fungicide resistance in Magnaporthe grisea.