禽巴氏杆菌抗链霉素耐药基因StrA的克隆及同原性比较

Cloning and Expression of Avian Pastuerella Multocida Streptomycin Resistance in Pronucleus

根据巴氏杆菌基因序列设计合成一对引物,以临床分离的动物源性巴氏杆菌抗链霉素耐药菌株为模板,通过PCR技术,扩增出StrA基因350-1153bp序列.PCR产物及表达载体通过粘端连接,将长约804bp的StrA目的基因片段克隆到pGEM-T载体上,构建了克隆载体质粒pGEM-TstrA.StrA基因片段测序结果与文献报道的结果同源性为99.8%.只有一个碱基发生突变,但所编码的氨基酸没有改变.

We designed and synthesised a pair of primer pastuerella gene rank,350-1153 rank of are amplified by pastuerella strain,PCR products bonded with expression vecter of plasmid carrier,804bp were cloned into pGEM-T vecter,constructing cloning vecter pGEM-TstrA.The sequences of StrA gene have 99.8% homology with that of the standard strain. Only one base occurrenced mutation,but coding amino acid weren't alterated.