Cyclin D1和VEGF在胃癌组织中的表达及其临床意义

Expression of Cyclin D1 And Vascular Endothelial Growth Factor in Gastric Carcinoma and its Signification

目的 探讨细胞周期蛋白D 1(cyclinD 1)和血管内皮生长因子 (VEGF)表达与胃癌分型、分期、浸润转移等生物学行为的关系。方法 应用免疫组织化学S P法 ,检测各期胃癌共 5 7例、慢性萎缩性胃炎 2 0例组织中cyclinD1和VEGF的表达。 结果 胃癌组cyclinD 1阳性表达率 ( 4 7.4% )高于慢性萎缩性胃炎组 ( 2 0 .0 % ) ;cyclinD1表达与肿瘤分化程度密切相关 ,与肿瘤浸润深度、肿瘤大小、淋巴结状态及TNM分期无关。胃癌组VEGF阳性表达率 ( 77.2 % )显著高于慢性萎缩性胃炎组 ( 5 .0 % ) ;VEGF的表达与胃癌的分化程度、肿瘤浸润深度、肿瘤大小、淋巴结状态、TNM分期均密切相关。在胃癌组织中 ,cyclinD1与VEGF的表达呈正相关。cyclinD 1、VEGF均阳性组以Ⅲb期和Ⅳ期患者多 ,cyclinD1、VEGF均阴性组以Ⅰa +Ⅰb和Ⅱ +Ⅲa期患者多。 结论 cyclinD1过表达参与了胃癌的发生 ,是胃癌发生过程中 1个较早期的分子事件。VEGF促进胃癌的增长 ,促使肿瘤向黏膜深层浸润并向远处转移。cyclinD 1和VEGF在胃癌的发生中起协同作用 ,其表达与TNM分期密切相关 。

Objective To investigate the relationship of the expression of cyclin D1,VEGF and the histological type,stage,infiltration and metastasis of gastric carcinoma(GC).Methods The expression of cyclin D1 and VEGF were tested by S-P immunohistochemistry in 57 cases of GC and 20 cases of chronic atrophy gastritis(CAG).Results The positive expression rate of cyclin D1 was higher in GC than in CAG.The expression of cyclin D1 were closely correlated to the differentiation degree of the tumor,but had no relation with tunor′s size?depth of infiltration,lymph node metastasis and TNM staging.The positive expression rate of VEGF was much higher in GC(77.2%) than in CAG(5.0%).The VEGF expression rate were closely correlated to tumor′s differentiation degree?tunor′s size?depth of infiltration,lymph node metastasis and TNM staging.There was a relationship between cyclin D1 and VEGF expression in GC(P=0.041,γ=0.264).Conclusion The excessive expression of cyclin D1 and VEGF may synergize in the carcinogenesis and development of GC.The combination detection of cyclin D1 and VEGF may be as a reference marker in the surgical treatment and the prognosis of GC.