骨巨细胞瘤组织中VEGF、NOS的表达及其与肿瘤血管形成的关系

Expression of VEGF,NOS in giant cell tumor of bone and their relation to angiogenesis

目的 检测VEGF,iNOS和eNOS在骨巨细胞瘤组织中的表达及其与肿瘤血管形成的关系。方法 应用免疫组织化学的方法,检测49例骨巨细胞瘤微血管密度(MVD)和VEGF,iNOS和eNOS在肿瘤组织中的表达。结果 VEGF、iNOS和eNOS的阳性表达率分别为61.2％,57.1％和71.4％。骨巨细胞瘤组织Jaffe病理分级间MVD比较,Ⅲ级>Ⅰ级(P<0.01),Ⅲ级>Ⅱ级(P<0.01),而Ⅰ级与Ⅱ级之间差异无显著意义(P>0.05)。VEGF阳性骨巨细胞瘤组织中MVD为37.7±10.5/高倍视野,VEGF阴性的骨巨细胞瘤组织中MVD为24.5/高倍视野±6.8/高倍视野,两者差异有显著性(P<0.01);iNOS阳性骨巨细胞瘤组织中MVD为37.9/高倍视野±10.9/高倍视野,iNOS阴性骨巨细胞瘤组织中MVD为25.2/高倍视野±7.1/高倍视野,两者差异有显著性(P<0.01);eNOS阳性骨巨细胞瘤组织中MVD为35.4/高俯视野±11.7/高信视野,eNOS阴性骨巨细胞瘤组织中MVD为25.6/高倍视野±5.8/高抬视野,两备差异有显著性(P<0.01);VEGF与iNOS表达均阳性者21例,VEGF表达阴性,而iNOS阳性者7例,二者表达有相关性(P<0.05)。VEGF与eNOS表达均阳性者19例,VEGF表达阴性,而eNOS阳性者16例,二者表达无相关性(P>0.05)。结论MVD是评估骨巨细胞瘤生物学行为的重要指标,VEGF,iNOS与eNOS在骨巨细胞瘤中有促进肿瘤血管形成的作用?

Objective To analyze the expression of vascular endothelial growth factor(VEGF), inducible nitric oxide synthase (iNOS) and endothelial nitric oxide synthase (eNOS) in giant cell tumor of bone (GCT) and their rela- tions to angiogenesis. Methods Expression of VEGF, iNOS and eNOS on tumor tissues from 49 cases of GCT patients were studied by immunohistochemistry. Microvessal density (MVD) was counted by endothelial cells immunostained by anti - CD34 anlibody. Results The positive expression of VEGF, iNOS and eNOS were observed in GCT and the posi- tive rates were 61.2 % , 57.1% , and 71.4% respectively . The MVD significantly correlated with pathological grade, Ⅲ>Ⅱ , Ⅲ>Ⅰ . MVD was 37.7 ±10.5/HP, 37.9±10.9/HP and 35.4 ±11.7/HP in GCT positive for VEGF, iNOS and eNOS while it was 24.5 ±6.8/HP, 25.2±7.1/HP and 25.6±5.8/HP in GCT negative for VEGF , iNOS and eNOS respectively( P<0.01 ) . There was significant correlation between iNOS and VEGF in GCT( P<0.05) , and no significant correlation between eNOS and VEGF in GCT ( P>0.05 ) . Conclusion The MVD is the most useful parame- ter to evaluate the biologic behavior of GCT. The angiogenesis of GCT was accelerated by the expression of VEGF, iNOS and eNOS.