黄芩甙治疗银屑病的机制研究

Study on the Mechanism of Baicalin in Psoriasis Treatment

目的探讨黄芩甙治疗银屑病的可能机制。方法 以体外培养的角质形成细胞、成纤维细胞、外周血单个核细胞为对象,不同浓度黄芩甙处理细胞后,用MTT比色分析法反映细胞增殖变化,流式细胞仪测定细胞周期分布。结果 1.5-96μg/mL黄芩甙对成纤维细胞显示一定的抑制作用,并呈时间和剂量依赖关系,而该浓度范围对良性角质形成细胞株HaCaT细胞无明显影响,且外周血单个核细胞活力在此浓度不受影响。流式细胞仪检测细胞周期分布变化,成纤维细胞随药物浓度的升高,G0-G1期细胞比例逐渐增高,G2M、S期细胞比例降低;而HaCaT无明显细胞周期分布变化。结论一定浓度的黄芩甙可通过阻滞成纤维细胞周期发挥抑制增殖作用,进而影响表皮角质形成细胞生长,是其治疗银屑病的可能机制之一。

Objectives To study the mechanisms of baicalin in psoriasis treatment. Methods Human benign epidermal keratinocytes (HaCaT cells), human fibroblasts were treated with different concentration of baicalin in vitro. The growth inhibition effects were determined by measuring MTT absorbance of living cells, and cell cycle distribution was detected by flow cytometry. Results Baicalin in concentration from 1.5 μg/mL to 96 μg/mL inhibited the proliferation of fibroblasts in a dose and time dependent manner, but it had no obvious effects on keratinocytes and peripheral blood mononuclear cells (PBMCs). Further study by DNA flow cytometric analysis revealed that cell percentage of fibroblasts, rather than HaCaT cells, in G0 to G1 phase increased and those in G2M, S phase decreased with the denser of baicalin' s concentration. Conclusion It suggested that baicalin could inhibit the proliferation of fibroblasts, further to affect the proliferation of keratinocytes, which was one of its mechanisms of psoriasis treatment.