期刊文献+

离体热损伤成纤维细胞所涉及的细胞信号通路 被引量:1

SIGNAL PATHWAY INVOLVED IN HEAT-STRESSED FIBROBLASTS
下载PDF
导出
摘要 利用已建立的成纤维细胞热损伤诱导凋亡模型观察离体培养的成纤维细胞热烫伤后信号通路的活化情况。首先将原代培养的人成纤维细胞经 5~ 6传代后 ,45℃水中孵育 10min,造成单纯热损伤刺激 ,加入 5 %小牛血清继续培养 ,分别在伤后 0、3 0、60和180min 4个时相点冻存细胞 ,运用Westernblot进行MAPKs信号通路磷酸化蛋白印迹检测 ,同时采用荧光显微镜检测热损伤成纤维细胞caspase3蛋白的表达情况。结果显示 ,经过热损伤刺激的成纤维细胞 ,c junN末端激酶 (JNK)首先开始表达 ,并在 60min达到高峰 ,能持续至 180min。细胞外信号调节激酶(ERK)的表达高峰在伤后 3 0min,随后迅速消退。损伤早期 ,成纤维细胞内caspase3阳性标记的细胞数较少 ,至伤后 60min ,caspase3的表达明显增强。研究表明 。 To explore the activities of signal transduction pathway involved in heat-stressed fibroblast in vitro. Human dermal fibroblasts were cultured in Dulbecco′s modified Eagle′s medium with 5% calf serum at 5%CO 2 in a water-saturated atmosphere. Cultured cells were heated to 45℃ for 10min. Western blotting was used to detect ERK1/2 and JNK expression, and the expression of caspase-3 protein was observed by immunoflurescence technique. The results showed that the MAPKs signal transduction pathway was activated in heat-stressed fibroblasts. The expression of JNK reached the peak at 60min, then maintained up to 180min. The expression of ERKs peaked at 30min, then lowered. The expression of caspase-3 was weak at 30min after heat-stress, and became evidently strong at 60min. The signal pathway of ERKs and JNK played important roles in the changes in biologic characteristics of fibroblasts after heat-stress.
出处 《解放军医学杂志》 CAS CSCD 北大核心 2003年第7期590-591,I004,共3页 Medical Journal of Chinese People's Liberation Army
基金 国家重点基础研究规划 (编号G1 9990 542 0 4 ) 国家自然科学基金重点项目 (编号 30 2 30 370 ) 国家杰出青年科学基金 (编号 3952 50 2 4 ) 国家自然科学基金 (编号 30 1 70 966) 博士后基金 (编号中博基 2 0 0 1 1 4 )资助课题
关键词 丝裂素活化蛋白激酶 创伤与损伤 信号传递 成纤维细胞 mitogen-activated protein kinases wounds and injuries signal transduction fibroblasts
  • 相关文献

参考文献8

  • 1程飚,付小兵,盛志勇,张萍,孟晋红.离体成纤维细胞热损伤模型制作的研究[J].中华创伤杂志,2002,18(7):391-393. 被引量:12
  • 2[2]Tekin D, Xi L, Zhao T et al. Mitogen-activated protein kinases mediate heat shock-induced delayed protection in mouse heart. Am J Physiol Heart Circ Physiol, 2001, 281(2): H523
  • 3[3]Beere HM. Stressed to death: regulation of apoptotic signaling pathways by the heat shock proteins. Sci STKE, 2001, 93(1): 1
  • 4[4]Davis RJ. Signal transduction by the JNK group of MAP kinases. Cell, 2000, 103(2): 239
  • 5[5]Jonak C, Okresz L, Bogre L et al. Complexity, cross talk and integration of plant MAP kinase signalling. Curr Opin Plant Biol, 2002, 5(5): 415
  • 6[6]Yates S, Rayner TE. Transcription factor activation in response to cutaneous injury: role of AP-1 in reepithelialization. Wound Repair Regen, 2002, 10(1): 5
  • 7[7]Cobb MH. MAP Kinase pathway. Pro Biophy Mol Biolog, 1999, 71(3-4):479
  • 8程飚,付小兵,盛志勇,张萍,孟晋红.丝裂原活化蛋白激酶通路对成纤维细胞内游离钙的影响[J].解放军医学杂志,2002,27(5):380-382. 被引量:6

二级参考文献7

共引文献14

同被引文献17

  • 1Rockwell WB, Cohen IK, Ehrlich HP. Keloids and hypertrophic scars:a comprehensive review [J]. Plast Reconstr Surg, 1989, 84 ( 5 ) : 827-837.
  • 2Bfissett AE, Sherris DA. Scar contractures, hypertrophic scars, and Reloids[J] .Facial Plast Surg,2001,17(4):263-272.
  • 3Gfinnen F, Zhu M, Carlson MA, et al. Release of mechanical tension triggers apoptosis of human fibroblasts in a model of regressing granulation tissue[J]. Exp Cell Res, 1999,248(2) :608-619.
  • 4Toplak H, Batchiulis V, Hermetter A, et al. Effects of culture and incubation conditions on membrane fluidity in monolayers of cultured cells measured as fluorescence anisotropyusing trimethy lammoniumdiph-enylhexatriene(TMA-DPH) [J]. Biochem Biophys Acta, 1990,1028(1):67-72.
  • 5Sojcic Z, Toplak H, Zuehlke R, et al. Cultured human skin fibroblasts modify their plasma membrane lipid composition and fluidity according to growth temperature suggesting homeoviscous adaptationat hypothermic(30 degrees C) but not at hyperthermic(40 degrees C) temperatures[J]. Biochem Biophys Acta, 1992,1104( 1 ) : 31-37.
  • 6Cherry GW, Wilson J. The treatment of ambulatory venous ulcer patients with warming therapy [J]. Wound Management, 1999,45 (9) : 65-70.
  • 7Volloch V, Gabai VL, Rits S, et al. HSP72 can protecte cells from heatinduced apoptosisby accelerating the inactivation of stress kinase JNK[J]. Cell Stress Chaoerone,2000,5(2) : 139-147.
  • 8Beere HM. Stressed to death : regulation of apoptotic signaling pathways by the heat shock nroteins[J]. Sci STKE. 2001.93 (1),1-7.
  • 9Berrnan B, Flores F. Comparison of a silicone gel-fined cushion and silicon gel sheeting for the treatment of hypertrophic or keloid scars[J].Dermatol Surg, 1999,25 (6) :484-486.
  • 10Poston J. The use of silicone gel sheeting in the management of hypertrophic and keloid scars[J]. J Wound Care, 2000,9(1) : 10-16.

引证文献1

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部