成人基底前脑巢蛋白免疫阳性神经细胞的细胞化学观察

THE CYTOCHEMICAI IDENTIFICATION OF THE NESTIN POSITIVE CELLS IN ADULT HUMAN BASAL FOREBRAIN

目的 探讨巢蛋白 (nestin)免疫阳性细胞在人基底前脑内的分布规律及化学特性。 方法 采用nestin 331B、10C2、Rat4 0 1抗体显示人基底前脑的nestin免疫阳性细胞 ,并应用NSE、p75NGFR、ChAT、GFAP抗体分别对nestin免疫阳性细胞进行了双标免疫细胞化学研究 ,同时还应用NADPH d进行组织化学染色。 结果 在成人基底前脑有一个连续的nestin免疫阳性细胞带 ,胞体较大 ,呈卵圆形或梭形 ,有 1～ 3个突起 ,分布于隔核、斜角带核、Meynert核、无名质及杏仁核群。双标染色显示 ,nestin阳性细胞被NSE抗体标记 ,并与ChAT、NGFR、NOS阳性神经元间杂分布 ,多数不呈交叉反应。约 2 8%nestin免疫阳性细胞为ChAT阳性神经元 ,15 %为NGFR阳性神经元 ,6 %为NOS阳性神经元。此外 ,透明隔及隔区靠近软膜处有少量nestin免疫阳性细胞 ,其形态与星形胶质细胞相似 ,不与GFAP有交叉免疫阳性反应。 结论 成人基底前脑存在一个有别于ChAT、NGFR、NOS神经元的nestin免疫阳性神经元簇 。

Objective To investigate the distribution and chemical character of nestin immunoreactive cells in the human basal forebrain. Methods We explored systematically the distribution of nestin immunoreactive cells throughout the human basal forebrain by nestin 331B, 10C2, Rat401 antibody.Furthermore, we investigated the chemical identity of these nestin immunoreactive cells by using double\|labeling immunocytochemistry with NSE, ChAT, p75NGFR, GFAP antibody or using NADPH\|d histochemistry. Results The nestin immunoreactive cells were found in the septum, diagonal band of Broca, innominate substance,amygdala,basal nucleus of Meynert of adult human brain.These nestin immunoreactive cells haven big cell body,2\|3 processes.The nestin immunoreactive cells were labeled by NSE antibody.The large majority of those were single stained,and 28% were double labeled with ChAT positive neurons,15% with NGFR positive neurons,6% with NOS positive neurons.A few nestin immunoreactive cells shared similar morphology with that of astrocyte glia which existed in the spaces between the thin septa pellucida or the midline along the septum.It could not be labeled by GFAP antibody.Conclusion\ A new cluster of nestin immunoreactive neurons that were different from the ChAT,NGFR,NOS positive neurons existed in adult human basal forebrain.\;[