ELISA法与RPR法检测梅毒抗体结果分析

Analysis of the results of detecting anti-Tp antibody by the method of ELISA and RPR in blood donor

目的 优选一种适宜于献血员梅毒筛查的试验方法。方法 采用检测梅毒特异性抗体的双抗原夹心(EL ISA)法对献血员进行抗体检测 ,并与 RPR法检测结果进行比较 ,对两种方法检测结果不一致的标本 ,再用 TPHA法进行确证。结果 EL ISA法阳性检出率 0 .37% (32 /84 6 2 )、RPR法阳性检出率 0 .2 7% (2 3/84 6 2 )。EL ISA法与TPHA总符合率 96 .87% (31 /32 )、RPR法与 TPHA总符合率 6 5 .6 2 % (2 1 /32 ) ,两者差异具有非常显著性 (χ2 =8.30 ,P<0 .0 1 )。结论 EL ISA法优于 RPR法 ,具有较高的灵敏度和特异性 ,适宜于献血员的筛查 。

Objective To select one kind of test method suitable for screening anti TP antibody in blood donor.Methods Anti TP antibody was screened by ELISA of double sandwich and compared with the results of RPR.TPHAmothod was then employed to confirm the different results of ELISA and RPR.Results The positive rate of ELISA was 0.37%(32/8 462) and RPR was 0.28%(23/8 462).The total coincidental rate between ELISA and TPHA was 96.87%(31/32),and the total coincidental rate between RPR and TPHA was 65.62%(21/32),which showed obvious difference between ELISA and RPR(χ 2=8.30, P <0.01).Conclusion The method of ELISA is better than RPR,the former is suitable for screening anti TP antibody in blood donor because of its higher sensibility and specificity and helpful to prevent and control syphilis from transmitting.