核磁共振技术在生物组织中的应用

APPLICATIONS OF NMR TECHNIQUES TO BIOLOGICAL TISSUES

在生物组织内部存在大量的生化、生理反应以及分子的扩散运动,它们相互独立,又相互关联,共同构成一个生化反应网络。在病理条件下,某些生化反应过程会发生改变,代谢中间体可能会出现质和量的变化,核磁共振(NMR)可以对这些过程的研究提供非常有用的信息。目前,有3种核磁共振波谱技术可以用于生物组织的研究:①活体组织定域波谱技术;②生物组织提取物的液体高分辨核磁共振技术;③离体组织的高分辨魔角旋转技术。这些方法各有优劣,互为补充,介绍了在生物组织的NMR研究方面的最新进展。

There are a great number of physiological and biochemical reactions inside biological tissues, which are either independent or correlated to each other. Useful information can be obtained by Nuclear Magnetic Resonance (NMR) technique in studying the metabolic processes. There are presently three kinds of NMR techniques for studying biological tissues. ① in vivo Magnetic Resonance Spectroscopy (MRS); ② High-Resolution in vitro Nuclear Magnetic Resonance (HR-NMR); ③ High-Resolution in vitro Magic Angle Spinning Nuclear Magnetic Resonance (HR-MAS NMR), each has its own advantages and disadvantages and are complementary to each other. In this article, progresses in the NMR studies of biological tissues are reviewed.