摘要
目的 :探讨有丝分裂原活化的蛋白激酶级联信号通路的关键效应分子——核糖体 S6蛋白激酶 (ribosom al S6 ki-nase,RSK)与肝纤维化发生的关系。 方法 :采用皮下注射二甲基亚硝胺 (dimethylnitrosamine,DMN)的方法制备大鼠肝纤维化模型 ,根据注射时间的不同 ,获取组织标本 ,行 H- E和 VG染色。明确纤维化分期后 ,进一步利用 Northern印迹及免疫组化方法 ,观察 RSK在肝纤维化进程中的表达变化情况。 结果 :RSK在正常大鼠肝脏中 ,仅在中央静脉周围的肝血窦有少量表达 ,而随着纤维化的进展 ,其表达量进行性增加 ;在纤维化肝脏 ,RSK主要分布于汇管区间质中 ,肝细胞未见染色。结论 :RSK在肝纤维化进程中持续上调 。
Objective:To investigate the relationship between expression of ribosomal S6 kinase (RSK),the key substrate of mitogen activated protein kinase cascades,and the development of hepatic fibrosis. Methods: Rat hepatic fibrosis were induced by subcutaneous injection of dimethylnitrosamine (DMN) and the rat livers were obtained for study after 1,2 and 3 weeks injection. The different stages of fibrosis were confirmed with H E and VG staining,and then the expression of RSK at transcription and translation level was detected by Northern blot and immunohistochemistry. Results: There was only weak expression of RSK in normal liver,and the expression increased accordingly with the development of fibrosis,which was mainly located within mesenchyme. Conclusion: RSK,up regulated in fibrotic liver,may play an important role in the pathogenesis of hepatic fibrosis.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2004年第2期153-156,共4页
Academic Journal of Second Military Medical University
基金
国家自然科学基金 ( 3 0 2 70 60 4)
