氟伐他汀对大鼠肺成纤维细胞增殖的抑制作用

Fluvastatin inhibiting proliferation of rat lung fibroblasts

目的 :观察氟伐他汀 (fluvastatin,Flu)对体外培养的大鼠肺成纤维细胞 (fibroblast,Fb)增殖的抑制作用。方法 :在体外培养的大鼠肺 Fb中 ,加入 Flu,用计数法和 MTT比色法观察并比较不同浓度的 Flu对培养细胞增殖的抑制作用、对大鼠肺 Fb分裂指数及分泌胶原的影响。 结果 :计数法和 MTT比色法均显示 ,Flu对体外培养的大鼠肺 Fb的增殖具有明显的抑制作用 (P<0 .0 1 ) ,并呈剂量依赖效应 ,1 0 - 4mol/ L 的 Flu已可使培养细胞的增殖受到完全抑制。培养的大鼠肺 Fb细胞分裂指数及胶原分泌量在 Flu的作用下也有明显降低 (P<0 .0 1 )。结论 :Flu对体外培养的大鼠肺 Fb的增殖及其胶原分泌具有明显的抑制作用 。

Objective:To observe the inhibitory effect of fluvastatin (Flu) on the proliferation of the rat lung fibroblasts cultured in vitro . Methods: Normal rat lung derived fibroblasts were cultured in media containing Flu. The influences of Flu on the growth curve of the fibroblasts were observed by cell count and MTT colorimetry. The inhibition effect of Flu serial dilutions on the fibroblasts proliferation was investigated. Influence of Flu on division index of the fibroblasts was analyzed by direct cell count. Chemical colorimetry was used to detect the hydroxyproline in the media. Results: Flu inhibited the proliferation of the normal rat lung fibroblasts in the manner of dose dependence( P <0.01). Flu (10 -4 mol/L) completely inhibited proliferation of the fibroblasts. Division index and collagen production of cultured fibroblasts also decreased obviously under the action of Flu( P <0.01). Conclusion: Flu can inhibit the proliferation of the normal rat lung fibroblasts in vitro , and it might be an effective medicine for pulmonary fibrosis.