应用微矩阵表达谱基因芯片筛选食管癌新的相关基因

Screening New Esophageal Cancer Associated Genes by cDNA Differentially Expressed Microarray

[目的]应用微矩阵表达谱基因芯片筛选食管癌新的相关基因。[方法]微矩阵表达谱基因芯片(14114种基因)筛选3例食管癌及其对照癌旁组织的差异表达基因,用Northern印迹证实,用末端终止法测序,将测序结果在GenBank数据库进行同源性检索。[结果]检测的3例临床标本中,共有的差异表达基因31条,上调基因10条,下调基因21条,其序列与Genbank数据库比较,从中筛选出2条无明显同源的人类已知基因或片段,即新基因,并列出其序列。[结论]微矩阵表达谱基因芯片可应用于筛选食管癌新的相关基因,其具备高通量、特异性好、快速的特点;食管癌和对照癌旁组织间存在2条新的差异表达基因。

To explore the differences in gene expression between esophageal cancer and cancer around tissue using cDNA microarray and screen new esophageal cancer associated genes.Differentially expressed genes were detected in cancer and cancer around tissue in3cases with esophageal cancer by Northern blotting.The sequences were measured by chain termination method.The result of sequence was homologous checked in GenBank(NLM in USA).In the samples from3cases,there were31differentially expressed genes,among them10up-regulated and21down-regulated.Compared with the data in GenBank2genes were not homologous.These sequences were listed.[Conclusion]With the characteristics of high flux,special and rapid,cDNA differentially expressed microarray is useful for searching the new gene.There are2new differentially expressed genes in esophageal cancer and cancer around tissue.