新型杯[4]芳烃高效液相色谱固定相的制备及表征

Preparation and Characterization of a New p-Allylcalix[4]arene-bonded Silica Gel Stationary Phase for High-Performance Liquid Chromatography

合成了一种新型的杯 [4 ]芳烃高效液相色谱键合固定相 ,考察了它的反相色谱行为 .通过对多环芳烃、二取代苯的位置异构体、苯甲酸酯和邻苯二甲酸酯的分离 ,发现该键合相具有显著的反相特征 ,对位置异构体具有很高的识别能力 ,其分离明显优于C1 8柱 。

For the first time calix[4]arene tetraamide bonded stationary phase was prepared by modifying silica gel with 3 mercaptopropyltriethoxysilane and coupling with p allylcalix[4]arene tetraamide. The separation of polycyclic aromatic hydrocarbons, positional isomers of nitroaniline, benzoic esters and phthalic esters on the new calix[4]arene bonded stationary phase was achieved with acetonitrile water or methanol water as mobile phase. A comparison with conventional reversed phase C 18 columns shows a predominant reversed phase character and obvious priority for separation of positional isomers. The possible separation mechanism has been investigated.