血管性血友病因子基因19内含子MspⅠ多态性与冠心病危险因素的关系

The relationship between von Willebrand factor gene MspⅠpolymorphism in intron 19 and risk factors for cardiovascular disease in Chinese

目的 :探讨血管性血友病因子 (vonWillebrandfactor ,vWF)基因 19内含子MspⅠ多态性与冠心病(CHD)危险因素的关系。方法 :应用聚合酶链反应 (PCR)技术结合MspⅠ酶切分析等 ,测定了 10 9例CHD患者和 10 4例健康对照者vWF基因 19内含子MspⅠ限制性片段长度多态性频率 ,同时应用酶联免疫吸附测定(ELISA)技术测定血浆vWF水平。结果 :①vWF基因 19内含子MspⅠ基因型与高血压间差异有统计学意义 ( P<0 .0 5 ) ,而与性别、年龄、糖尿病、高脂血症和吸烟等危险因素差异无统计学意义 (P >0 .0 5 )。②vWF基因 19内含子MspⅠ基因型在高危组和低危组的分布差异无统计学意义 (P >0 .0 5 )。③伴高血压患者中M-/M-基因型与M+/M+基因型和M+/M-基因型的血浆vWF水平之间无统计学意义 (P >0 .0 5 )。结论 :vWF基因 19内含子MspⅠ多态性可能与CHD的危险因素之一高血压有关。

Objective:To ascertain the relationship between von Willebrand factor gene MspⅠpolymorphism in intron 19 and risk factors for cardiovascular disease in Chinese. Methods:MspⅠ polymorphism was studied by PCR and MspⅠ digestion in 109 cases with CHD and 104 controls simultaneously. Risk factors of cardiovascular disease were assessed by standard questionnaire, physical examination and blood tests. Simultaneously, plasma vWF levels were measured by ELISA.Results:① There were no significant differences among genotypes in terms of sex, age, diabetes mellitus, hyperlipidemia and cigarette smoking in von Willebrand factor gene MspⅠpolymorphism (P> 0.05 ), as to hypertension, the difference was significant (P< 0.05 ). ② The MspⅠ genotypes distribution in high risk group and low risk group were not significantly different from each other (P> 0.05 ).③The plasma vWF levels of M -/M -, M +/M + and M +/M - genetypes in hypertension subgroup were ( 180.25 ± 83.23 )% and ( 178.76 ± 91.94 )%, respectively (P> 0.05 ).Conclusion: von Willebrand factor gene MspⅠpolymorphism in intron 19 may be associated with hypertension in Chinese.