复方花刺参粘多糖对家兔动脉损伤后血脂及动脉内膜的影响

Effects of stichopus variegatus compound on blood lipids and hyperplasiaof arterial tunica intima in atherosclerotic rabbits

目的 :探讨复方花刺参粘多糖对家兔动脉损伤后血脂、动脉内膜厚度的影响及作用机制。方法 :雄性新西兰大白兔 37只随机分为假手术组、模型组、花刺参组和辛伐他汀组 ,髂动脉损伤术 6周处死全部家兔取血及受损动脉 ,检测各组家兔的血清脂质浓度 ,苏木精 伊红染色观察动脉形态学改变 ,图像分析测量动脉内膜、中膜厚度 ,原位杂交检测生长因子 [转化生长因子 β1(TGF β1)、成纤维细胞生长因子 (bFGF)和血小板衍生长因子(PDGF)表达。结果 :花刺参组血清总胆固醇 (TC)及低密度脂蛋白胆固醇 (LDL C)浓度显著低于模型组 (P <0 .0 5 ) ;花刺参组和辛伐他汀组动脉内膜厚度、内膜 /中膜厚度比及PDGF及TGF β1的表达明显少于模型组 (P<0 .0 1) ;花刺参组与辛伐他汀组各项参数之间比较均差异无统计学意义。结论 :复方花刺参粘多糖能有效的调整血脂 ,减轻家兔髂动脉损伤血管内膜厚度和管腔狭窄 。

Objective:To investigate the effects of Stichopus Variegatus Compound on blood lipids and thickness of intima in rabbits and its mechanisms.Methods:Thirty seven male New Zealand white rabbits were allocated randomly into 4 groups: normal control, modle group, Stichopus group, Simvastatin group. All rabbits were killed after 6 weeks and iliac arteries were removed. In every group, rabbit's lipids including TC, TG, HDL C, LDL C, apoAⅠ, apoB and Lp(a) were observed. With HE staining, artery morphology alterations were observed and the thickness of arterial intima and media were measured. The expression of growth factor bFGF, TGF β 1 and PDGF were observed by hybridization in situ. Results:The serum concentration of TC and LDL in Stichopus group was significantly lower than that in modle group (P< 0.05 ). The thickness of intima and I/M in treatment group decvealed significantly compared with that in modle group (P< 0.01 ).The expression of PDGF and TGF β 1 in treatment group decrealed significantly compared with that in modle group (P< 0.05 ).There were no significant differences between Simvastation group and Stichopus group.Conclusion:Stichopus Variegatus Compound can effectively regulate lipids, especially significantly decreasing TC and LDL C concentration. Stichopus Variegatus Compound can relieve stenosis of blood vessel, and it can effectively inhibit hyperplasia of smooth muscle cell, which partly attributed to its function of inhibiting the synthesis and excretion of growth factors.