摘要
Objective To investigate the role of cyclin-kinase inhibitor p27 on proliferation of mesangial cell(MC) induced by tumor necrosis factor α( TNF-α ). Methods The p27 protein of MC lysate was detected with western blotting analysis. The degree of MC proliferation was estimated through [^3H] thymidine incorporation. The effect of reducing p27 expression on MC proliferation was analysed with p27 antisense oligodeoxynucleotide (ODN). Results TNF-α(200000U/L) decreased p27 level to (0.6±0.1 ) from (1. 1±0.1 ) of MC lysate cultured in serum free DMEM for 24h ( P<0.01 ) and increased [^3H] thymidine incorporation to ( 2060±112 ) from (685±53) cpm/well( P<0.01 ). p27 antisense ODN transfection decreased p27 level of MC stimulated by TNF-α for 24h [(0.3±0.1 ) vs (0.6±0.1), P <0.01 ] and increased [^3H] thymidine incorporation [(2420±130) vs (2060±112) cpm/well, P <0.05]. Conclusion The decline of p27 protein maybe play an important role in MC proliferation induced by TNF-α.
Objective To investigate the role of cyclin-kinase inhibitor p27 on proliferation of mesangialcell(MC) induced by tumor necrosis factor α(TNF-α). Methods The p27 protein of MC lysate was detected with western blotting analysis. The degree of MC proliferation was estimated through [3H]thymidine incorporation. The effect of reducing p27 expression on MC proliferation was analysed with p27 antisense oligodeoxynucleotide (ODN). Results TNF- α(200000U/L) decreased p27 level to (0.6±0.1) from (1.1±0.1) of MC lysate cultured in serum free DM EM for 24h (P <0.01) and increased [3H] thymidine incorporation to (2060±112) from (685±53) cpm/well(P <0. 01). p27 antisense ODN transfection decreased p27 level of MC stimulated by TNF-αfor 24h [(0.3±0.1) vs (0. 6±0. 1) , P < 0. 01 ] and increased [3H] thymidine incorporation [ (2420±130) vs (2060±112) cpm/well, P <0. 05]. Conclusion The decline of p27 protein maybe play an important role in MC proliferation induced by TNF-α.
