摘要
Objective To demonstrate the differently expressed genes between proliferating and involuting hemangiomas by cDNA microarray in an effort to clarify the mechanism of conversion from proliferating hemangioma to involution one. Methods Samples of the proliferating and involuting phases in the same hemangioma were processed from total RNA to purified mRNA , then reverse-transcripted and hybridized onto BioDoor human 4096 target genes microarray. Analyses were performed to determine the consensus pattern of gene expression and their changes in expression level. Results In proliferating hemangioma, 79 genes were underexpressed, and 115 genes were overexpressed in comparison with that of involuting hemangioma as control. Differently expressed genes may be related to the process of conversion in that. ( 1 ) Some cytokines and growth factors were overexpressed in proliferating hemangioma ; ( 2 ) In involuting hemangioma, apoptotic factors were overexpressed ; (3) Angiogenesis and oncogenes might participate in the pathogenesis of hemangiomas ; ( 4 ) Mitochondral activated apoptotic passage and Wnt/beta-catenin passage were involved. Conclusion The altered gene expression profile between proliferatingand involuting phase of the same hemangioma shows that the development of hemangiomas may be the results of imbalance of cell proliferation and apoptosis.
Objective To demonstrate the differently expressed genes between proliferating and involutinghemangiomas by cDNA microarray in an effort to clarify the mechanism of conversion from proliferating hemangioma to involution one. Methods Samples of the proliferating and involuting phases in the same hemangioma were processed from total RNA to purified mRNA, then reverse-transcripted and hybridized onto BioDoor human 4096 target genes microarray. Analyses were performed to determine the consensus pattern of gene expression and their changes in expression level. Results In proliferating hemangioma, 79 genes were underexpressed, and 115 genes were overexpressed in comparison with that of involuting hemangioma as control. Differently expressed genes may be related to the process of conversion in that. (1) Some cytokines and growth factors were overexpressed in proliferating hemangioma; (2)In involuting hemangioma,apoptotic factors were overexpressed; (3)Angiogenesis and oncogenes might participate in the pathogenesis of hemangiomas;(4)Mitochondral activated apoptotic passage and Wnt/beta-catenin passage were involved. Conclusion The altered gene expression profile between proliferating and involuting phase of the same hemangioma shows that the development of hemangiomas may be the results of imbalance of cell proliferation and apoptosis.
