P73基因在喉鳞癌中的表达及其临床意义

Expression of P73 gene of laryngeal squamous cell carcinoma and its clinical significance

目的 探讨P73基因在喉鳞癌中的表达。方法 用原位杂交方法检测 4 9例喉鳞癌组织、19例声带息肉、12例癌旁组织及 8例非典型增生组织中P73的表达。结果 喉鳞癌组织P73阳性表达 2 6例定位于胞浆 ,9例定位于腺体 ,5例定位于胞核 ,9例未表达。P73阳性表达率在喉鳞癌组织、声带息肉、癌旁组织及非典型增生组织中分别为 5 3.0 6 % (2 6 / 4 9) ,36 .84 % (7/ 19) ,4 1.6 7% (5 / 12 ) ,37.5 % (3/ 8) ,P73与喉鳞癌的临床分型、分期无相关性。结论 P73基因可能并非喉癌癌变过程中的靶基因 ,在喉癌的发生发展中可能不发挥重要作用。

Objective To explore the correlation between the gene P73 and the laryngeal squamous cell carcinoma (LSCC). Methods The genetic expression of P73 in 49 cases of LSCC, 19 cases of vocal cord polyp, 12 cases of paracancerous tissue, and 8 cases of nontypical hyperplasia tissue were examined by insitu hybridization histochemical method. Results The positive genetic expression of P73 in 26 cases was located in cytoplasm, 9 cases in gland, 5 cases in nucleus, and 9 cases not expressed. The positive expression rates of P73 in LSCC tissues, polyps of the vocal cord tissues, paracancerous tissues, and nontypical hyperplasis tissues were 53.06% (26/49), 36.84% (7/19), 41.67% (5/12) and 37.5% (3/8) respectively(P> 0.05 ). The gene P73 was not correlated with the clinical stages and clinical typing of LSCC. Conclusion The gene P73 might not be a target gene in laryngeal carcinogenesis and not play a major role in development of LSCC.