PCR检测鸭疫里默氏菌的研究

Detection of Riemerella Anatipestifer by PCR

对本研究室分离、鉴定并保存的42株鸭疫里默氏菌(包括8个血清型)、9株鸭源大肠杆菌和4株鸭源禽多杀性巴氏杆菌进行PCR扩增,结果所有的鸭疫里默氏菌均能扩增出809bp的特异性DNA片段,而鸭源大肠杆菌和鸭源禽多杀性巴氏杆菌均为阴性。试验证明以细菌DNA和全菌体分别作模板,对PCR的扩增结果无影响。经过优化的该PCR方法能检测出的最低DNA量为1pg。

Polymerse chain reaction technique was developed to detect 42 isolates of R.anatipestifer(RA) which belonged to 8 serotypes. The result showed that a 809 bp DNA fragment as it was expected was ampilified from all detected R.anatipestifer strains, while 9 Escherichia coli strains and 4 Pasteurella multocida strains isolated from duck gave negative results. It showed no difference whether whole bacteria cells or DNA of RA was used as the template for the PCR .The lowest amount of DNA of RA detectable by this PCR-based test was 1pg.